AIM: To study the alteration of the content of guanine nucleotide binding protein α subunits ( G α protein) in myocardium during shock, and the effect of naloxone ( NAL) on the G protein. 目的:研究失血致低血容量性休克大鼠心脏组织鸟苷酸结合蛋白(Guaninenucleotide-bindingprotein,G蛋白)含量的变化及纳络酮对其影响。
Guanine nucleotide binding proteins ( G proteins), known as an important signal transduction molecular, can physiologically couple extracellular signals identified by G protein coupled receptors ( GPCRs) to a series of intracellular effector to cause changes of gene transcription and protein's structure and function. G蛋白是一类重要的信号转导分子,其生理功能是将细胞膜受体所识别的各种细胞外信号同细胞内一系列效应分子偶联起来,引起核基因转录及蛋白质结构和功能的变化。
The subunit α_0 of guanine nucleotide-binding protein, in the areas of rat brain and spinal cord was localized by immunohistochemical methods. 本研究应用兔抗鸟核苷酸调节蛋白α0亚单位(简称α0)多克隆血清(抗体),采用免疫组织化学方法对大鼠脑和脊髓内一些结构中的α0作了定位观察。
It was shown by evidence that ( 1) there were two classes of guanine nucleotide binding sites on the Gp of calf testis membranes, high affinity ( with low capacity) binding sites and low affinity ( with high capacity) binding sites; 证据表明,①在小牛睾丸细胞膜G蛋白上存在两种类型鸟嘌呤核苷酸结合位点(下简称GTP结合位点),高亲和性低容量结合位点及低亲和性高容量结合位点;
Small GTPases are monomeric guanine nucleotide binding proteins coupled to the α-subunit of heterotrimeric G proteins. All small GTPases belong to Ras superfamily. 小G蛋白是和异三聚体G蛋白α亚基偶联的单体鸟苷酸结合蛋白,所有的小G蛋白从属于Ras超家族。
Small GTPase proteins play a pivotal role as important molecular switches, containing four guanine nucleotide ( GTP/ GDP) binding ( GNB) domains and an effectors domain. 小GTPase蛋白作为重要的分子开关其结构域主要包括4个鸟苷酸(GTP/GDP)结合域及1个效应区。